Heterogeneous template-dependent transcription dynamics of T7 RNAP revealed by single-molecule imaging

Proceedings of the National Academy of Sciences, Volume 122, Issue 50, December 2025. SignificanceBacteriophage T7 RNA polymerase is a key tool in RNA synthesis, but its efficiency can be hindered by premature stalls and low yields. This study sheds light on the causes of these limitations: the flexibility and electrostatic potential of …